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Fig. 5 | Nutrition & Metabolism

Fig. 5

From: Low-arginine and low-protein diets induce hepatic lipid accumulation through different mechanisms in growing rats

Fig. 5

VLDL secretion from the livers, lipogenesis, fatty acid uptake, and RERs of rats fed the 5PAA and low Arg diets. Rats were fed the 15PAA (n = 8), 5PAA (n = 8), and low Arg (n = 8) diets. After 5 h of feeding, rats were injected with tyloxapol (200 mg/kg), and then TG concentrations (a and c) were measured at 0, 30, 60, 120, and 240 min post-injection. The RER (b) of each rat (n = 6) was measured for 24 h. A de novo lipogenesis assay (d) was performed by measuring palmitate isotopomers after intraperitoneal injection of D2O or H2O into rats fed the 15PAA (n = 8), 5PAA (n = 8), and low Arg (n = 8) diets for 1 night (18:00–10:00). Fatty acid uptake (e) was evaluated by incorporation of a fatty acid analog, BODIPY® FL C12, into the livers of rats fed the 15PAA (n = 4), 5PAA (n = 4), and low Arg (n = 4) diets for 1 night (18:00–10:00), and fluorescence was measured in the liver lipid extractions. The values are the means ± standard errors. Asterisks indicate significant differences assessed by one-way ANOVA with Bonferroni and Tukey–Kramer post-hoc tests (*p < 0.05)

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