Table 2 PCR reaction conditions
Genes | Initial denature | Cycle parameters | Final extension | Cycle number |
|---|---|---|---|---|
COXI | 94 °C 5 min | 94 °C 30 s; 60 °C 30 s; 72 °C 45 s | 72 °C 10 min | 25 |
PGC-1α | 94 °C 5 min | 94 °C 30s; 64 °C 45 s; 72 °C 45 s | 72 °C 10 min | 30 |
UCP2 | 94 °C 5 min | 94 °C 30s; 55 °C 30 s; 72 °C 1 min | 72 °C 10 min | 30 |