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Table 2 PCR reaction conditions

From: Endogenous asymmetric dimethylarginine accumulation contributes to the suppression of myocardial mitochondrial biogenesis in type 2 diabetic rats

Genes Initial denature Cycle parameters Final extension Cycle number
COXI 94 °C 5 min 94 °C 30 s; 60 °C 30 s; 72 °C 45 s 72 °C 10 min 25
PGC-1α 94 °C 5 min 94 °C 30s; 64 °C 45 s; 72 °C 45 s 72 °C 10 min 30
UCP2 94 °C 5 min 94 °C 30s; 55 °C 30 s; 72 °C 1 min 72 °C 10 min 30