Early leucine programming on protein utilization and mTOR signaling by DNA methylation in zebrafish (Danio rerio)

Table 1 Primers of all genes for qRT-PCR are listed

	Sequence 5′-3′	Tm (°C)
accα	F: GTGGAAACAAAGTTATTGAGAAGG	55
accα	R: GTAAGCCCAGCGTCGGA	55
cpt1	F: ATCAGCACTGTTGAGCGAAG	59
cpt1	R: CACTCCCTCCCTACTTATCTCC	59
cs	F: TTCGCTCGGGCGTATTCT	59
cs	R: GCTGCTGCCTTCACGGTAT	59
fas	F: GATGGACGAGTGCTTTACCC	55
fas	R: ATGGTGGCTCTATGGATGGT	55
got	F: GCTAAAGGCTTACACCTACTAT	56
got	R: GTCAAAGAACACCAGGAGAT	56
gpt	F: AGAAGACCCTGACGATGGAC	56
gpt	R: GAGGAAGGTGATTGGTTGCT	56
Pfk1	F: AACGAACTCTTCCAAACTCCTG	55
Pfk1	R: GACTCCTTCATACGCCTCAAAT	55
mtor	F: GCCGCTTTGCCAACTATTT	55
mtor	R: TCGTCTGCCTTCATTCCTG	55
leptinA	F: ATTCCCGCTGACAAACCC	56
leptinA	R: GTAACCCAGAAGTGTGGATAGATC	56
leptinB	F: CCCCGTCACCTCCAACTACCT	59
leptinB	R: CAGAGAATGAATGTCTCAGCCACA	59
grb10	F: TCCGAACCCTTTCCCTGAG	60
grb10	R: CTTCCACAACTTTTCCCACA	60
wdr24	F: TTACTGAGCGGCAAACCC	57
wdr24	R: TGATTCGCAGCATCGTCC	57
eif4e	F: AGTGATGATGTCTGTGGTGCTG	61
eif4e	R: TGTTCTCGTAGTCTGTCGTCC	61
β-actin	F: CACCTTCCAGCAGATGTGGA	58
β-actin	R: AAAAGCCATGCCAATGTTGTC	58

ISSN: 1743-7075