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Fig. 4 | Nutrition & Metabolism

Fig. 4

From: High intensity exercise downregulates FTO mRNA expression during the early stages of recovery in young males and females

Fig. 4

Expression levels of FTO, AS160 Ser588 phosphorylation, and phosphorylated AMPK in the vastus lateralis muscle. a Representative Western Blots of pAS160Ser588, total AS160, pAMPK, total AMPK, FTO and Actin for each genotype measured during pre-exercise rest (R), and at 10 mins and 90 mins during passive recovery following HI intensity exercise. b & c Relative expression of FTO protein when normalised to Actin following HI and LO intensity exercise respectively. d Relative expression of AS160 Ser588 phosphorylation (pAS160Ser588) relative to total AS160 following HI intensity exercise. e Relative expression of phosphorylated AMPK relative to total AMPK following HI intensity exercise. Data are expressed as mean ± SEM of the fold change at 10 and 90 mins compared to pre-exercise measurements (set arbitrarily to 1). ‘a’ represents significant main effect for time (p < 0.05)

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