Fig. 4From: High intensity exercise downregulates FTO mRNA expression during the early stages of recovery in young males and femalesExpression levels of FTO, AS160 Ser588 phosphorylation, and phosphorylated AMPK in the vastus lateralis muscle. a Representative Western Blots of pAS160Ser588, total AS160, pAMPK, total AMPK, FTO and Actin for each genotype measured during pre-exercise rest (R), and at 10 mins and 90 mins during passive recovery following HI intensity exercise. b & c Relative expression of FTO protein when normalised to Actin following HI and LO intensity exercise respectively. d Relative expression of AS160 Ser588 phosphorylation (pAS160Ser588) relative to total AS160 following HI intensity exercise. e Relative expression of phosphorylated AMPK relative to total AMPK following HI intensity exercise. Data are expressed as mean ± SEM of the fold change at 10 and 90 mins compared to pre-exercise measurements (set arbitrarily to 1). ‘a’ represents significant main effect for time (p < 0.05)Back to article page