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Fig. 2 | Nutrition & Metabolism

Fig. 2

From: High-protein and low-calorie diets improved the anti-aging Klotho protein in the rats’ brain: the toxic role of high-fat diet

Fig. 2

Molecular assessment of Klotho-α and FGF23 in the hippocampus and prefrontal cortex of experimental groups. Rats treated with high-fat, high-protein, low-calorie, low-calorie high-protein diets for 10 weeks. Control adult and old rats treated with rodent standard pellet. After behavioral tests, rats were euthanized and brains of three of them perfused for histological evaluation in each group (n = 3) and brains of other three rats in each groups were collected for Western blotting technique (n = 4). Immunoflorence assay a showed the Klotho-α distribution in the hippocampus and prefrontal cortex. Mean of fluorescence intensity showed Klotho-α positive cell in the CA1 and prefrontal cortex (b). A represented blot showed Klotho-α and FGF23 protein level (c) and the density of Klotho-α bands (d) and FGF23 band (e) were measured in the hippocampus and prefrontal cortex (n = 4 and technical repeat for each n is 3). Data were presented as Mean ± S.D. ***P < 0.001 ver. Adult-C, #P < 0.05, ##P < 0.01, ###P < 0.001 ver. Old-C. $P < 0.05 ver. High-protein. Adult-C adult control rats, Old-C old control rats

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