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Fig. 3 | Nutrition & Metabolism

Fig. 3

From: Sialylated human milk oligosaccharides prevent intestinal inflammation by inhibiting toll like receptor 4/NLRP3 inflammasome pathway in necrotizing enterocolitis rats

Fig. 3

SHMOs pre-treatment promoted proliferation, reduced mitochondrial damage and altered MMP-2 activity of Caco-2 cells. a Viabilities of Caco-2 cells co-cultured with different doses of LPS (0, 1, 2, 5, 10, 20 μg/mL) for 20 h. b Cell viabilities of Caco-2 cells pre-treated with different doses of SHMOs (500, 1500 or 4500 mg/L) for 1 h, followed by co-cultured with LPS (0, 10 or 20 μg/mL) for another 20 h. c Cell cycle, d mitochondrial membrane potential and e MMP-2 activity of Caco-2 cells pre-treated with pooled SHMOs (1500 mg/L) for 1 h, followed by co-cultured with LPS (20 μg/mL) for another 20 h. Data were presented as mean ± SD (n = 5 for a and b, n = 3 for ce). *p < 0.05; **p < 0.01; ***p < 0.001 analyzed by one-way ANOVA followed by Turkey’s multiple comparisons test

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