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Table 3 Assessment of the bias of 25(OH)D2 detected by LC-MS/MS using NIST certified SRM-972a

From: Determination of serum 25-hydroxyvitamin D status among population in southern China by a high accuracy LC-MS/MS method traced to reference measurement procedure

SRM-972a
25(OH)D2
Certified values by NIST LC-MS/MS
Target values (ng/mL) Uncertainty Detected values (ng/mL) SD (n = 3) Bias(%)
Level 1 0.50 0.06 0.48 0.07 −4.17
Level 2 0.81 0.06 0.84 0.05 4.45
Level 3 12.90 0.30 12.72 0.26 −1.40
Level 4 0.50 0.10 0.51 0.04 2.26
  1. SRM standard reference material, NIST National Institute of Standards & Technology