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Fig. 8 | Nutrition & Metabolism

Fig. 8

From: Vaspin antagonizes high fat-induced bone loss in rats and promotes osteoblastic differentiation in primary rat osteoblasts through Smad-Runx2 signaling pathway

Fig. 8

Effects of vaspin on osteogenic protein expression of primary rat osteoblasts. After treated with different concentrations of vaspin protein expression of Runx2, Osx, β-catenin (a), Smad2, Smad3, p-Smad2, and p-Smad3 (b) in primary rat osteoblasts was detected by Western blot analysis. The relative protein levels were quantified by densitometry and normalized to GAPDH(c-g). Data are expressed as the mean ± SD from three independent experiments. *p < 0.05 compared with control group, #p < 0.05 compared with 10 ng/ml vaspin group, ##p < 0.05 compared with 50 ng/ml vaspin group

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