Vaspin antagonizes high fat-induced bone loss in rats and promotes osteoblastic differentiation in primary rat osteoblasts through Smad-Runx2 signaling pathway

Table 1 Primer sequences used for the determination of gene expression

Gene (Rats)	Primer sequence((5′ - 3′)
Colla1	Forward GCGAAGGCAACAGTCGCT
Colla1	Reverse CTTGGTGGTTTTGTATTCGATGAC
Runx2	Forward CCTGAACTCAGCACCAAGTCCT
Runx2	Reverse TCAGAGGTGGCAGTGTCATCA
Osx	Forward CTGGGAAAAGGAGGCACAAAGA
Osx	Reverse GGGGAAAGGGTGGGTAGTCATT
β-actin	Forward TGCTATGTTGCCCTAGACTTCG
β-actin	Reverse GTTGGCATAGAGGTCTTTACGG

ISSN: 1743-7075