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Fig. 2 | Nutrition & Metabolism

Fig. 2

From: Tetramethylpyrazine reverses high-glucose induced hypoxic effects by negatively regulating HIF-1α induced BNIP3 expression to ameliorate H9c2 cardiomyoblast apoptosis

Fig. 2

Hypoxia aggravates High-glucose induced cellular apoptosis via BNIP3 regulation. a Flow cytometry analysis show aggravated cell death in H9c2 cells up on exposure to hypoxia under high-glucose challenge. The results also show reversal of hypoxia-high glucose induced cell death in the presence of siBNIP3 (n = 3). The cells without labeling represents living (Q3) cells; the cells labeled with Annexin V-FITC (Q2, Q4) or propidium iodide (Q1, Q2) were respectively cells in apoptosis and in necrosis (b) Western blotting analysis show the correlation of BNIP3 expression with caspase 8 activation (n = 3) up on hypoxia exposure in high glucose condition

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