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Fig. 3 | Nutrition & Metabolism

Fig. 3

From: Oleic acid ameliorates palmitic acid induced hepatocellular lipotoxicity by inhibition of ER stress and pyroptosis

Fig. 3

Palmitic acid induced pyroptosis activation,and oleic acid protected HepG2 cell against pyroptosis. HepG2 were treated with 0.4 mM PA, 0.2 mM OA or their combination (PA/OA). a. The mRNA expressions of key genes governing pyroptosis were detected after 24 h treatment, and β-ACTIN was used as an internal control. b. HepG2 cells were stained with anti-GSDMD (red) antibody and DAPI, and then visualized under a microscope at 200× magnification after 24 h treatment. c. Cell supernatants were analyzed for IL-1β secretion by ELISA. d and e. Representative western blots of NLRP3, GSDMD/−N, pro-CAS-1, P20 and IL-1β after 24 h treatment, and β-ACTIN was used as a protein-loading control. f. The morphology of pyroptotic cells was visualized by AO/EB staining. All groups were visualized under a microscope at 50× and 200× magnification after 24 h treatment. The data are presented as means ± SD for 3–5 biological replicates; **P < 0.01, ***, P < 0.001vs. BSA;#P < 0.05,##P < 0.01,vs. PA; ns no significant differences between two connected groups

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