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Fig. 1 | Nutrition & Metabolism

Fig. 1

From: Ferulic acid alleviates lipotoxicity-induced hepatocellular death through the SIRT1-regulated autophagy pathway and independently of AMPK and Akt in AML-12 hepatocytes

Fig. 1

Ferulic acid protects hepatocytes against palmitate-induced cell death. a AML-12 cells were treated with different concentrations (0, 50, 100, 200, 400, and 800 μM) of ferulic acid (FA). After 24 h of incubation, cell viability was analysed by MTT assay. b, c AML-12 cells at 80% confluence were incubated with 0.5 mM palmitic acid (PA) for 12 h after pretreatment for 2 h with different concentrations (0, 25, 50, and 100 μM) of FA. Cell death was measured by LDH release in the culture medium and MTT assay. d Total cellular lysates were probed for cleaved-caspase-3 by immunoblotting. e The nuclear morphology was detected by Hoechst staining. All values are shown as the means ± SD from three or more independent batches of cells. Bars with different superscripts are significantly different at p ˂ 0.05

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