Fig. 3

The effect of long-chain monounsaturated fatty acids on β-cell viability. INS-1E (a) cells were treated with vehicle [0 µM] or 250 µM C16:0, or C18:1 either alone or in combination [500 µM] for 24 h. EndoC-βH1 cells were exposed to vehicle [0 µM] or 500 µM C16:0 or C18:1 (b) for 72 h. Cell death was assessed using flow cytometry after staining with propidium iodide. Dots represent individual data points from a minimum of three independent experiments and the histograms represent mean values + SEM. ***p < 0.001 relative to vehicle [0 µM]