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Fig. 4 | Nutrition & Metabolism

Fig. 4

From: Lotus leaf extract inhibits ER breast cancer cell migration and metastasis

Fig. 4

Hydrogen peroxide level in LAE-inhibited cell migration. a The content of intracellular H2O2 was determined after treating with 100 µg/ml LAE for 48 h in SK-BR-3, MDA-MB-231 and HCC1806. b NOX4, NOX2, catalase and SOD1 proteins levels were examined by immunoblotting with different concentrations of LAE (0, 10, 25, 50, 100, 250 µg/ml). c Wound healing assays after treating with LAE alone or combined with 25 nM H2O2 for 36 h (× 100 magnification). d The content of intracellular H2O2 was determined after treating with 100 µg/ml LAE alone or combined with TGF-β1 (10 ng/ml) for 48 h in SK-BR-3, MDA-MB-231 and HCC1806. Values represent the mean ± SD (n = 3)

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