Fig. 4

Expression of tight junction-associated proteins and genes and Notch signaling pathway-related proteins and genes in SW480 cells. 1: control group; 2: LPS group; 3: VD₃ group; 4: 0.1VC + VD₃ group; 5: 1VC + VD₃ group; 6: 5VC + VD₃ group; 7: 10VC + VD₃ group. a Western blotting results of claudin-2 in SW480 cells, b western blotting results of ZO-1 in SW480 cells, c western blotting results of Notch intracellular domain (NICD) in SW480 cells, d western blotting results of Hes-1 in SW480 cells, e RT-PCR of tight junction protein-related genes and Notch signaling pathway-related genes. In the figure, a–f indicate significant differences compared with the control group, LPS group, VD₃ group, 0.1VC + VD₃ group, 1VC + VD₃ group, and 5VC + VD₃ group, respectively