Table 4 Polymerase chain reaction primers' gene sequences for homosapiens
Species | Target gene | Primer sequence | Length (bp) |
|---|---|---|---|
Homosapiens | β-Actin | Forward:GAGAAAATCTGGCACCACACC | 177 |
Reverse:GGATAGCACAGCCTGGATAGCAA | |||
GAPDH | Forward:CCTGCACCACCAACTGCTTA | 176 | |
Reverse:GGCAGGGATGATGTTCTGGA | |||
Notch1 | Forward:TGAATGGCGGGAAGTGTGAAG | 219 | |
Reverse:CATTGTCCAGGGGTGTCAGG | |||
Hes-1 | Forward:AAAAATTCCTCGTCCCCGGT | 194 | |
Reverse:TGCCGCGAGCTATCTTTCTT | |||
Claudin-2 | Forward:TATGTCGGTGCCAGCATTGT | 257 | |
Reverse:GCTACCGCCACTCTGTCTTT | |||
ZO-1 | Forward:AGCCATTCCCGAAGGAGTTG | 199 | |
Reverse:AGGATCACCGTCAGGAGTCA |