Fig. 3

Microarray analysis of BAT in the different groups of mice and validation. Clusters of the differentially expressed genes in the HC versus NC groups (a1) and the HE versus HC groups (a2). Green represents downregulation relative to the mean level of the control group, and red represents upregulation. Black indicates missing or excluded data. GO analysis of the differentially expressed genes (b1, b2, b3 and b4). Comparison of the top biological process (BP) associated with the differentially expressed genes in the HC versus NC (HC/NC) and HE versus HC (HE/HC) groups. The figures show the BP enriched in the top five differential genes (ranked by input gene number) in each group. HC/NC↑/↓ indicates that the HC group was higher/lower than the NC group, and HE/HC↑/↓ indicates that the HE group was higher/lower than the HC group, both here and in the following. c Wayne diagram of the major pathways enriched in the differentially expressed genes. Red (region and text) represents genes that are upregulated in the HC/NC group, and green represents genes that are downregulated. Orange represents genes that are upregulated in both HC/NC and HE/HC groups downregulated. The white text depicts genes whose levels of expression did not differ. d The interaction diagram and the COX2 control network diagram of the differentially expressed genes. The differentially expressed genes in the red frame have a primary regulatory relationship with Ptgs2/COX2; the orange frame contains the secondary regulatory genes, while the green frame contains the tertiary regulatory genes. e RT-qPCR verification of key differentially expressed genes. *P < 0.05 and **P < 0.01 represent statistical significance. n = 3