Aerobic exercise promotes the functions of brown adipose tissue in obese mice via a mechanism involving COX2 in the VEGF signaling pathway

Table 2 Pathway analysis of the differentially expressed genes (P < 0.05)

Comparison	Major Enriched Pathways	Associated Genes
HC versus NC↑	Jak-STAT signaling pathway	Prlr, Pik3r5, Pim1, Cntfr, Csf2rb, Il22ra2, Il10ra
	TNF signaling pathway	Cx3cl1, Icam1, Pik3r5, Map2k6, Ifi47, Edn1, Il1b, Ccl5, Mapk13, Mmp9
	NF-kappa B signaling pathway	Il1b, Lbp, Icam1, Bcl2a1d, Ccl4
HC versus NC↓	Arachidonic acid metabolism	Ptgr1, Ptgis, Cyp4a14, Ptges
HC versus NC↓	Insulin signaling pathway	Fasn, Acaca, Pik3r5, Mtor, Fbp1, Ppp1r3c, Srebf1, Hras
HE versus HC↑	Arachidonic acid metabolism	Cyp1a1, Cyp4a32, Ptges, Cyp4a31, Cyp4a10, Ptgr1, Ptgis, Cyp4a14, Ptgs2
	AMPK signaling pathway	Lep, Pfkfb3, Ccnd1, Pfkl, Pik3r5, Pfkp, Scd2, Prkab1, Fasn, Fbp1, Scd3, Gys2, Acaca, Ppp2r5b, Pik3r1, Irs2
	PPAR signaling pathway	Me1, Scd3, Ehhadh, Hmgcs2, Cyp4a32, Cyp4a31, Fabp5, Cyp4a10, Acsl5, Cyp4a14, Acox2, Scd2
	Insulin signaling pathway	Pik3r1, Fasn, Acaca, Pde3a, Prkab1, Fbp1, Pik3r5, Gys2, Hk1, Hk2, Cblc, Irs2
	VEGF signaling pathway	Pik3r5, Pik3r1, Nos3, Mapkapk3, Ptgis, Ptgs2
HE versus HC↓	Jak-STAT signaling pathway	Lep, Myc, Ccnd1, Pik3r5, Pik3r1, Cish, Cntfr, Il22ra2, Cblc
	ErbB signaling pathway	Zfyve28, Cdh13, Cblc, Btc
	TGF-beta signaling pathway	Myc, Cdkn2b, Id2, Acvr1c, Skp1a, Bmp8b, Bmpr1b, Tgfbr2

Janus kinases/signal transducer and activator of transcription (JAK/STAT); Tumor necrosis factor (TNF); Nuclear factor-kappa B (NF-kappa B); Adenosine 5’-monophosphate (AMP)-activated protein kinase (AMPK); Receptor tyrosine-protein kinase erbB isoform b (ErbB); Peroxisome proliferator-activated receptor (PPAR); Vascular endothelial growth factor (VEGF); Transforming growth factor-beta (TGF-beta). n = 3

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