Fig. 4

DHM administration promoted the activation of hepatic NK cells in mice. a–c The frequency of intra-hepatic NK cells (cells were gated by NK1.1⁺ CD3⁻) and NKG2D expression (cells were gated by NK1.1⁺ CD3⁻ NKG2D⁺) which in intra-hepatic NK cells were analyzed by FCM. d–e Frequencies of intra-hepatic NK cells and IFN-γ expression (cells were gated by NK1.1⁺ CD3⁻ IFN-γ ⁺) in intra-hepatic NK cells were analyzed by FCM. Experiments were independently repeated at least three times. *p < 0.05, **p < 0.01, compared to the control group; ^#p < 0.05, ^##p < 0.01, compared to the CCl₄ group. FCM, flow cytometry; IFN-γ, interferon gamma; NK cells, natural killer cells; NKG2D, NK cell receptor natural killer group 2 member D