Fig. 4
DHM administration promoted the activation of hepatic NK cells in mice. a–c The frequency of intra-hepatic NK cells (cells were gated by NK1.1+ CD3−) and NKG2D expression (cells were gated by NK1.1+ CD3− NKG2D+) which in intra-hepatic NK cells were analyzed by FCM. d–e Frequencies of intra-hepatic NK cells and IFN-γ expression (cells were gated by NK1.1+ CD3− IFN-γ +) in intra-hepatic NK cells were analyzed by FCM. Experiments were independently repeated at least three times. *p < 0.05, **p < 0.01, compared to the control group; #p < 0.05, ##p < 0.01, compared to the CCl4 group. FCM, flow cytometry; IFN-γ, interferon gamma; NK cells, natural killer cells; NKG2D, NK cell receptor natural killer group 2 member D