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Fig. 3 | Nutrition & Metabolism

Fig. 3

From: Calcium channel blockers do not protect against saturated fatty acid-induced ER stress and apoptosis in human pancreatic β-cells

Fig. 3Fig. 3

Effect of diazoxide, nifedipine, and verapamil on the effect of stearic acid (SA) on the level of cleaved caspase-9 (C9), caspase-8 (C8), caspase-7 (C7), caspase-6 (C6), and cleaved PARP (markers of apoptosis) and on the level of BiP and CHOP (markers of ER stress) in NES2Y and 1.1B4 cells. After 24 h of incubation, the levels of individual proteins were determined using western blot analysis and the relevant antibodies (see “Materials and Methods”). Monoclonal antibody against human actin was used to confirm equal protein loading. The data shown were obtained in one representative experiment from two till six independent experiments. DMSO was used as vehicle. Densitometric analysis of key data from Western blotting where perceptible change in the expression was detected is also shown. Each column represents the mean of at least two experimental values ± SEM. * p < 0.05 when comparing the effect of respective inhibitor + 1 mM SA with the effect of 1 mM SA applied alone. D300 means 300 µM diazoxide, N100 means 100 µM nifedipine and V100 means 100 µM verapamil

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