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Fig. 2 | Nutrition & Metabolism

Fig. 2

From: Influence of HFD-induced precocious puberty on neurodevelopment in mice

Fig. 2

HFD feeding affects hypothalamic- pituitary–gonadal axis. a Effects of HFD after weaning in early follicular development(HE 100x). b, c Representative photomicrographs of kisspeptin1 and kisspeptin1 receptor immunostaining of ovaries. d, e MOD for kisspeptin1 and kisspeptin1 receptor were summarized. f, g Quantification of the levels of protein to kisspeptin1 and kisspeptin1 receptor in hypothalamus by western blotting. hi Quantification of the levels of protein to GnRH and GnRH receptor in hypothalamus by western blotting. j Serum LH and FSH in two groups. k Serum estradiol was increased in HFD group. Data are means ± SEM. *P < 0.05, **P < 0.01, and ***P < 0.001

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