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Fig. 1 | Nutrition & Metabolism

Fig. 1

From: Androgen receptor regulates the proliferation of myoblasts under appropriate or excessive stretch through IGF-1 receptor mediated p38 and ERK1/2 pathways

Fig. 1

Under un-stretched state, AR deficiency slowed down the proliferation of myoblasts. a The difference in the level of AR and b cell number between C2C12 and L6 myoblasts were determined by western blot and cell counting, respectively. *p < 0.05 vs C2C12 myoblasts at the corresponding time point. c AR overexpression plasmid (2.5 ug/well) was transfected into L6 myoblasts, and the level of AR protein was determined and compared with C2C12 cells, and d cell proliferation was detected by CCK8. *p < 0.05, the proliferation of L6 myoblasts transfected with pcDNA3.1-AR vs pcDNA3.1 vector; e siRNA-AR (100 pmol/well) was transfected into C2C12 myoblasts using Lipofectamine 2000 reagent, and the level of AR protein was determined and compared with L6 myoblasts, and f cell proliferation was detected. *p < 0.05, the proliferation of C2C12 myoblasts transfection with siRNA-AR vs siRNA-ctrl. The experiments were repeated at least three times, and the results were from three independent experiments and represented as mean ± SD

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