Fig. 4

AR overexpression in L6 myoblasts reversed 20% stretch-induced anti-proliferation. L6 myoblasts were seeded onto flexible-bottomed 6-well plates and divided into four groups: CON plus transfected with pcDNA3.1, CON plus transfected with pcDNA3.1-AR, 20% stretch plus transfected with pcDNA3.1, and 20% stretch plus transfected with pcDNA3.1-AR groups. The cells were transfected with pcDNA3.1 vector or pcDNA3.1-AR recombinant plasmid (2.5 μg/well) before exposing to 20% stretch. Then the level of AR protein and cell proliferation were detected at 24 h after stretch finished by Western blot and CCK8, respectively. The results were from three independent experiments and represented as mean ± SD, *p < 0.05 vs CON_pcDNA3.1; ^#p < 0.05 vs 20% stretch_pcDNA3.1