Fig. 6
The decreased extents of activations in p38, ERK1/2 and PI3K/Akt were different between 20% stretched L6 and C2C12 myoblasts a and transfection L6 cells with AR overexpression plasmid reversed the above molecules' activities (b and c). a C2C12 and L6 cells were seeded onto flexible-bottomed 6-well plates and incubated for 24 h prior to 20% stretch. Then cells were collected and the activities of PI3K/Akt, p38 and ERK1/2 (reflected as the ratios of p-PI3K/PI3K, p-Akt/Akt, p-p38/p38 and p-ERK1/2/ERK1/2, respectively) were detected by Western blot at 24 h after stretch finished. The decreased extents in activities of PI3K, p38 and ERK1/2 were calculated using the following formula: = (the activities of PI3K/Akt and MAPKs (p38 and ERK1/2) 20% stretch—the activities of PI3K/Akt and MAPKs (p38 and ERK1/2) CON)/ the activities of PI3K/Akt and MAPKs (p38 and ERK1/2) CON. * indicated p < 0.05 vs. C2C12 cells. b and c L6 myoblasts were seeded onto flexible-bottomed 6-well plates, when the confluence reached ~ 50%, pcDNA3.1 vector or pcDNA3.1-AR overexpression plasmid was transfected into L6 myoblasts before subjected to 20% stretch, and the activities of PI3K/Akt, p38 and ERK1/2 were detected at 24 h after stretch. *p < 0.05 vs. CONpcDNA3.1; #p < 0.05 vs 20% stretchpcDNA3.1