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Fig. 8 | Nutrition & Metabolism

Fig. 8

From: Androgen receptor regulates the proliferation of myoblasts under appropriate or excessive stretch through IGF-1 receptor mediated p38 and ERK1/2 pathways

Fig. 8

Exogenous IGF-1 recombinant polypeptide further promoted the proliferation of 15% stretched L6 myoblasts, accompanied with the increases in the protein level of IGF-1R as well as the activities of PI3K/Akt and MAPKs (p38 and ERK1/2) in a dose-dependent manner. L6 myoblasts were divided into five groups (CON, 15% stretch, and 15% stretch administrated with 200, 500 and 1000 ng/ml IGF-1 recombinant polypeptide), and IGF-1 recombinant polypeptide at different concentrations were added into L6 cell culture medium at 1 h prior to 15% stretch, and cell proliferation were detected by CCK8 (A); the protein level of IGF-1R (B), and the activities of PI3K/Akt (C) and MAPKs (D) were detected by Western blot at 24 h after stretch finished. The results were from three independent experiments, and the data were analyzed and represented as mean ± SD. *p < 0.05 vs. CON; #p < 0.05 vs 15% stretch

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Nutrition & Metabolism

ISSN: 1743-7075

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