Fig. 4

DHM attenuates PA-induced autophagy arrest and oxidative stress in hepatocytes partially through ATG4B. The HepG2 cells with ATG4B expression (HepG2/ATG4B^+/+) and silencing ATG4B (HepG2/ATG4B^+/−) were treated as indicated. A–C The expression of P62 and LC3 were analyzed by western blotting (A). And the bar graphs showed the quantification of P62 (B) and LC3 (C). D, E The intracellular ROS levels were measured by FCM assay (D) and quantified (E). F, G The mtROS levels were analyzed by FCM (F) and quantified (G). H, I The MMP levels were measured by FCM assay (H) and quantified (I). Graphs showed mean ± SEM; n = 3; data were from one experiment out of three. *p < 0.05, **p < 0.01, ***p < 0.001, compared between the marked groups; ns no significant difference