Fig. 5

DHM facilitates SIRT3-mediated deacetylation of ATG4B in hepatocytes. A, B Western blotting analysis of total protein acetylation using an anti-acetyllysine antibody (AcK) and the protein expression of SIRT3 (A). The bar chart showed the quantification (B). C The expression of ATG4B followed by co-immunoprecipitated with the anti-SIRT3 antibody was analyzed by western blotting. D, E HepG2 cells were pretreated with 3-TYP (50 μM) and then treated with PA (0.2 mM). The expressions of the acetylated and total ATG4B in HepG2 cells were measured by western blotting (D). The densitometric quantification of the ratio of acetylated-ATG4B/ATG4B (E). F, G HepG2 cells were treated with DHM and followed by PA treatment. The expressions of the acetylated and total ATG4B in HepG2 cells were analyzed by western blotting (F). The bar graph showed the quantification of the ratio of acetylated-ATG4B/ATG4B (G). Graphs showed mean ± SEM; n = 3; data were from one experiment out of three. *p < 0.05, **p < 0.01, ***p < 0.001, compared between the marked groups; A.U. arbitrary units