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Fig. 2 | Nutrition & Metabolism

Fig. 2

From: L-carnitine ameliorates the muscle wasting of cancer cachexia through the AKT/FOXO3a/MaFbx axis

Fig. 2

L-carnitine activates the AKT/FOXO3a pathway, increases p70S6k protein expression, and promotes p70S6k phosphorylation in vitro. A The expression levels of total AKT and p-AKT in the C2C12 myotubes were determined by Western blotting at different time points after 1000 μg/ml L-carnitine treatment (0, 2, 4, 8, 16, 24 h) following stimulation with TNF-α (100 ng/mL) for 2 h. B C2C12 myotubes were stimulated with TNF-α (100 ng/mL) for 2 h and then treated with different concentrations (100 or 1000 μg/mL) of L-carnitine for 16 h, and then the expression levels of total AKT and p-AKT were determined by Western blotting. C The expression levels of total p-p70S6K, p70S6K, p-FOXO3a and total FOXO3a were determined by Western blotting. D C2C12 myotubes were stimulated with TNF-α (100 ng/mL) for 2 h and then treated with different concentrations (100 or 1000 μg/mL) of L-carnitine for 16 h, and then the expression levels of total p-p70S6K, p70S6K, p-FOXO3a and total FOXO3a were determined by Western blotting

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