Fig. 3

A Orthogonal partial least squares discriminant analysis (OPLS-DA) of metabolites (LPS: n = 5, NS: n = 5). B Spot of OPLS-DA. C Volcano map of differential metabolites. D Correlation analysis of differential metabolites. E Heat map of differential metabolites; Red indicates up-regulated genes and blue indicates down-regulated ones. F KEGG enrichment of differential metabolites. G Contents of bile acids in serum. H Expression of metabolic enzymes in primary bile acid synthesis. *P < 0.05; **P < 0.01; ***P < 0.0001. FXR Farnesoid X-activated receptor, SHP short heterodimer partner, CYP7A1 cytochrome P450 family 7 subfamily A member, CYP27A1 cytochrome P450 family 27 subfamily A member 1, CYP8B1 cytochrome P450 family 8 subfamily B member, Srebp1a sterol regulatory element binding protein