Fig. 4
Expression of cleaved caspase-3 and DNA damage following treatments. The expression of cleaved caspase-3 (A) and DNA damage (B) in tumours following different treatments determined immunohistochemically using monoclonal antibodies. Quantitative analysis of the fluorescence intensity of cleaved caspase-3 (C) and DNA damage (D) expression in tumour tissues following various treatments determined by immunohistochemistry. Scale bar = 50 µM. Magnification = 40X. N = 6 mice per group. The level of fluorescence was measured using 8 randomly taken images that provide a total area of 1 mm2. The results are expressed as mean ± SEM; *P < 0.05, **P < 0.01, ***P < 0.001 compared to the sham group; ^^^P < 0.001 compared to the oxaliplatin-treated group. E The expression of cleaved caspase-3 and DNA damage measured by western blotting in tumours following various treatments