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Fig. 2 | Nutrition & Metabolism

Fig. 2

From: Anti-obesity effects of heat-transformed green tea extract through the activation of adipose tissue thermogenesis

Fig. 2

Co-treatment with HTGT and EMIQ synergistically increased mitochondrial content and activity in adipocytes in vitro. A Immunoblot analysis of adipocytes differentiated from immortalized brown preadipocytes treated with vehicle control (CTL), HTGT (100 μg/mL), EMIQ (100 μg/mL), or combination of HTGT and EMIQ (H + E, 50 μg/mL + 50 μg/mL) for 48 h (n = 6). B Immunoblot analysis of adipocytes differentiated from immortalized brown preadipocytes treated with vehicle control (CTL) or different ratio of combination of HTGT and EMIQ (1:1 (50 μg/mL + 50 μg/mL), 1:3 (25 μg/mL + 75 μg/mL), 3:1 (75 μg/mL + 25 μg/mL)) for 48 h (n = 6). C Oxygen consumption rate (OCR) analysis of adipocytes differentiated from immortalized brown preadipocytes treated with vehicle control (CTL) or combination of HTGT and EMIQ (H + E, 50 μg/mL + 50 μg/mL) for 48 h (n = 3). D MitoTracker staining of adipocytes differentiated from C3H10T1/2 cells treated with vehicle control (CTL) or HTGT (100 μg/mL), EMIQ (100 μg/mL), combination of HTGT and EMIQ (H + E, 50 μg/mL + 50 μg/mL) for 48 h (Size bar = 50 μm) (n = 3)

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