Fig. 5

Co-treatment with HTGT and EMIQ upregulated mitochondrial content and activity in adipose tissue in vivo. A, B Immunoblot analysis of brown adipose tissue (BAT) and inguinal white adipose tissue (iWAT) treated with vehicle (CTL), mirabegron (Mira, 10 mg/kg), or combination of HTGT and EMIQ (H + E, 200 mg/kg, 100 mg/kg each) or 100 mg/kg (50 mg/kg each)) for 2 weeks (n = 6). C Representative images UCP1 immunostaining of paraffin sections from iWAT of mice treated with vehicle (CTL), mirabegron (Mira, 10 mg/kg) or combination of HTGT and EMIQ (H + E, 100 mg/kg each) for 2 weeks (Size bar = 50 μm). D Triphenyltetrazolium chloride staining of BAT, iWAT and gonadal white adipose tissue (gWAT) from mice treated with vehicle (CTL), mirabegron (Mira, 10 mg/kg) or combination of HTGT and EMIQ (H + E, 200 mg/kg (100 mg/kg each) for 2 weeks (n = 6)