Fig. 3

MgIG ameliorate acute alcohol induced-hepatomegaly in zebrafish Larvae. a Tg(lfabp10a:eGFP) zebrafish larvae were treated as indicated for 32 h and the typical pictures of each group were shown. Arrows point to the liver. Control group: 0% alcohol; EtOH group: 350 mM alcohol; EtOH + MgIG(0.1) group: 350 mM alcohol + 0.1 mg ml⁻¹ MgIG; EtOH + MgIG(0.05) group: 350 mM alcohol + 0.05 mg ml⁻¹ MgIG;EtOH + MgIG(0.01)group: 350 mM alcohol + 0.01 mg ml⁻¹ MgIG; EtOH + NAC(20) group: 350 mM alcohol + 20 µM NAC. b Images of Tg(lfabp10a:eGFP) zebrafish larvae treated with 0% alcohol, 350 mM alcohol, pre-treated with 0.1 mg ml⁻¹ MgIG, 0.05 mg ml⁻¹ MgIG, 0.01 mg ml⁻¹ MgIG, 20 µM NAC and co-exposed with 350 mM alcohol for 32 h. The morphological change of livers in zebrafish larvae were observed. Alcohol-treated larvae developed obvious hepatomegaly compared to the control group after 32 h of exposure, which was visibly ameliorated in MgIG-pretreated larvae. c The liver size was examined by image J software in zebrafish larvae exposed to 0% or 350 mM alcohol and larvae pre-treated with different concentrations of MgIG, 20 µM NAC and then co-exposed with 350 mM alcohol for 32 h. The liver size was normalized to control group. *P < 0.05, **P < 0.01, n.s.: no significant difference, by one-way ANOVA