Fig. 6

L-theanine promotes fatty acid β oxidation through promoting the expressions of PPARα and CPT1A. HepG2 and AML12 cells were treated with 500 μM OA for 24 h with or without pretreated L-theanine for 2 h. Western blot analysis showing protein expression of PPARα and CPT1A in HepG2 (A) and AML12 (B) cells. C57BL/6J mice were fed normal control diet (NCD), HFD or HFD supplemented with L-Theanine for 16 weeks. C mRNA expression of Ppara and Cpt1a in mice liver. D Protein levels of PPARα and CPT1A in mice liver. E Representative images of immunohistochemical staining of PPARα and CPT1A in liver sections. Scale bar:50 μm (40 ×). Band intensity was quantified by densitometry analysis. Values are expressed as mean ± SEM of three independent experiments. *p < 0.05, **p < 0.01 versus control or NCD group; ^#p < 0.05, ^##p < 0.01 versus OA or HFD group