Fig. 6

Influences of miR-128-3p overexpression or SIRT1 silence on the effects of Sal B on PA-mediated ATDC5 cells. ATDC5 cells were transfected with miR-128-3p mimic or SIRT1 siRNA, and treated with Sal B (100 μM, 24 h) followed by the treatment of PA (500 μM, 24 h). A Relative expression of SIRT1 was determined by Western blot assay. B Cell viability, C–E Concentrations of pro-inflammatory cytokines in the culture supernatant, F and G Apoptotic ratio, and H and I expression levels of autophagy-related proteins were measured by MTT assay, ELISA assay, Flow cytometry analysis and Western blot, respectively. n = 3. Data are expressed as means ± SEM. Student’s t test was used for the comparison between 2 groups in this study. *P < 0.05