Fig. 7

JAK2/STAT3 pathway inhibition weakens the effects of Sal B on PA-mediated ATDC5 cell autophagy and apoptosis. ATDC5 cells were treated with Sal B (100 μM, 24 h), followed by the treatment of PA or JSI-124 (10 μM, 24 h). A The protein levels of p-JAK2, JAK2, pSTAT3, STAT3 were determined by Western blot. B Apoptotic ratio was detected by Flow cytometry analysis. C Relative expression levels of autophagy-related proteins were measured by Western blot. n = 3. Data are expressed as means ± SEM. One-way ANOVA was used for the comparison between 2 groups in this study. *P < 0.05