Table 1 Primer sequences and annealing temperatures
Gene | Sequence | Tm-annealing °C | |
|---|---|---|---|
NRF2 | F | TGTGGGGTAAGTCGAGAAGTG | 58 |
R | TACTGGGCTGGCTGAATTGG | ||
FGF21 | F | TTCGGGACTGTGGGTCTGTCTC | 61.5 |
R | TTTGCAGGTGGGCTTCGGTG | ||
MAFA | F | TCGTCAACGACTTCGACCTG | 58 |
R | TCAGAAGCTGGGCGAAGAG | ||
PDX1 | F | TTCCGGACCTTTCCCGAATG | 59 |
R | TTCTGCTGCGTATGCACCTC | ||
Î’-KLotho | F | TGCATTGGTTCTGCTGCGAG | 59 |
R | TAAATGCTCCGGTCCCAACG | ||
GAPDH | F | TCATCAACGGCACAGTCAAGG | 60 |
R | TTCTGCATGGTGGTGAAGACG | ||