Fig. 6

Western Blot of selected liver proteins related to glucose metabolism. A Western blot analyses of G6PC, PCK1, FBP1, SIRT1 and β-actin proteins (n = 6). B G6PC, PCK1, FBP1 and SIRT1 protein levels were quantified by densitometric analysis using ImageJ software (n = 6). C Western blot analyses of p-CRTC2, CRTC2, p-CREB, CREB, CBP and β-actin proteins (n = 6). D p-CRTC2, CRTC2, p-CREB, CREB and CBP protein levels were quantified by densitometric analysis using ImageJ software (n = 6). E Western blot analyses of p-FoxO1, FoxO1, p-PI3K, p-AMPK and β-actin proteins (n = 6). F p-FoxO1, FoxO1, p-PI3K and p-AMPK protein levels were quantified by densitometric analysis using ImageJ software (n = 6). G Western blot analyses of PGC-1a, GLUT2, GCKR and β-actin proteins (n = 6). H PGC-1a, GLUT2 and GCKR protein levels were quantified by densitometric analysis using ImageJ software (n = 6). Data are shown as mean ± SEM. **P < 0.01, ***P < 0.001. BCAA: branched-chain amino acids, CON: control, G6PC: glucose-6phosphatase, PCK1: phosphoenolpyruvate-carboxykinase 1, FBP1: fructose-1,6-bisphosphatase, SIRT1: Sirtuin 1, CRTC2: CREB regulated transcriptional coactivator 2, CREB: cAMP response element binding protein, CBP: CREB-binding protein, FoxO1: Forkhead box O1, PI3K: phosphatidylinositol 3 kinase, AMPK: AMP-activated protein kinase, PGC1a: Peroxisome proliferator-activated receptor gamma coactivator-1 alpha, GLUT2: glucose transporter 2, GCKR: glucokinase regulatory protein