Table 2 Details of primary antibody used in the experiment
Antibodies | Source | Catalogue no | Dilution ratio |
|---|---|---|---|
PGC1α | Abcam | ab191838 | 1:1000 |
CRTC2 | Proteintech | 12497-1-AP | 1:500 |
p-CRTC2 (Ser171) | Abcam | ab203187 | 1:500 |
SIRT1 | CST | 9475S | 1:1000 |
GLUT2 | Abcam | ab192599 | 1:1000 |
p-FoxO1 (Ser256) | CST | 9461T | 1:1000 |
FoxO1 | CST | 2880T | 1:1000 |
CBP | CST | 7389S | 1:1000 |
G6PC | Abcam | ab133964 | 1:1000 |
FBP1 | Abcam | ab109732 | 1:1000 |
p-AMPK(α1 + α2) | Abcam | ab133448 | 1:1000 |
p-PI3K(Y607) | Abcam | ab182651 | 1:1000 |
PCK1 | Abcam | ab70358 | 1:1000 |
CREB | CST | 9197S | 1:1000 |
p-CREB (Ser133) | CST | 9198S | 1:1000 |
GCKR | CST | 14328S | 1:1000 |
p-AKT(Ser473) | CST | 4051S | 1:1000 |
p-AKT(Thr308) | CST | 2965S | 1:1000 |
AKT | CST | 9272S | 1:1000 |
p-P70S6K(Thr389) | CST | 9206s | 1:1000 |
β-actin-HRP | Abways | AB2001 | 1:5000 |