Fig. 2

ACE2 deletion induced adipocyte hypertrophy, increased lipogenesis and ER stress related markers in epididymal adipose tissue in mice. A Hematoxylin/eosin staining for epididymal adipose tissue from WT and ACE2 KO mice. Bars indicate a length of 50um. B The mean diameter of epididymal adipocytes. C and D Representative Western blot and relative protein levels of lipogenesis related markers in epididymal adipose tissue. E and F Representative Western blot and relative protein levels of ER stress related markers in epididymal adipose tissue. Data are represented as mean ± SEM from n = 3. *p < 0.05, **p < 0.01, ***P < 0.001vs WT group. WT, wild-type; ACE2, angiotensin-converting enzyme 2; KO, knock out; FAS, fatty acid synthase; ACCα, acetyl-CoA carboxylase α; M-SREBP-1c, mature sterol regulatory element-binding protein-1c; GRP78, glucose regulated protein 78; ATF4, activating transcription factor 4; CHOP, C/EBP homologous protein