Fig. 6

Ang(1–7) treatment induced decreased adipocytes size, lipogenesis and ER stress related markers in epididymal adipose tissue of db/db mice. A Hematoxylin/eosin staining for epididymal adipose tissue from db/db mice treated with NS, Ang(1–7), and Ang(1–7) combined with A779. Bars indicate a length of 50um. B The mean diameter of epididymal adipocytes. C and D Representative Western blot and relative protein levels of lipogenesis related markers in epididymal adipose tissue. E and F Representative Western blot and relative protein levels of ER stress related markers in epididymal adipose tissue. Data are represented as mean ± SEM from n = 3. *p < 0.05, **p < 0.01, ***p < 0.001 versus NS group; ^#p < 0.05, ^##p < 0.01, ^###p < 0.001 versus Ang(1–7) group by one-way ANOVA. Ang(1–7), angiotensin(1–7); NS, normal saline; FAS, fatty acid synthase; ACCα, acetyl-CoA carboxylase α; M-SREBP-1c, mature sterol regulatory element-binding protein-1c; GRP78, glucose regulated protein 78; ATF4, activating transcription factor 4;CHOP, C/EBP homologous protein