Fig. 1

HUVECs treat with OGD over different time periods (0, 3, 6 and 9 h) showing that the degree of apoptosis increases with time. A Brightfield (40 × field) observations showed the growth and distribution of HUVECs after different OGD treatment times, n = 3/group. B The absorbances (OD values) of each group after different OGD treatment times were measured by CCK8 assays, n = 5/group. C Annexin V-FITC/PI reagent was used to detect apoptosis of HUVECs after different OGD treatment times by flow cytometry, n = 3/group. D The level of the apoptotic marker protein cleaved Caspase3 was measured by western blotting, n = 3/group. One-way ANOVA with multiple comparisons was utilized to determine the statistical significance as follows: *p value < 0.05, **p value < 0.01, and ***p value < 0.001