Fig. 10

Arginine metabolism pathway-related proteins changed significantly in HUVECs after different OGD treatment periods (0, 3, 6 and 9 h). A–D Intracellular mRNA levels of ASS1, ARG2, ODC1 and SAT1 were measured by RT‒PCR, n = 3/group. E Western blotting results of four metabolism-related proteins (ASS1, ARG2, ODC1 and SAT1), n = 3/group. F Quantitative analysis results of the four proteins. One-way ANOVA with multiple comparisons was utilized to determine the statistical significance as follows: *p value < 0.05, **p value < 0.01, and ***p value < 0.001