Fig. 2

HUVECs showed different levels of oxidative stress and early apoptosis after different times of OGD treatment. A The mitochondrial membrane potentials of the four groups of cells were observed by JC-1 detection under 585 nm emission and 514 nm excitation with a fluorescence microscope (× 100 field), n = 3/group. B Graphical representation of the green/red fluorescence intensity ratios in JC-1 detection. C ROS immunofluorescence assays were used to observe the production of ROS in cells (× 200 field), n = 3/group. D Fluorescence intensity value of DCF compound reflecting ROS content. One-way ANOVA with multiple comparisons: *p value < 0.05, **p value < 0.01, and ***p value < 0.001