Figure 5
Expression of substance P (SP) in the duodenum, jejunum and ileum of rats from DIO and DR groups (n = 6). Expression of SP in duodenum, jejunum and ileum of rats was examined by immunohistochemistry. Positive expression of SP showed a brown color. Integral optical density (IOD) of positive SP expression was quantified by image analysis. DIO: the group of diet–induced obesity; DR: the group of diet-induced resistance. Scale bar = 50 μm. **p < 0.01, compared to the DIO group.