Figure 9
Effects of sudachitin on gene expression in mouse primary skeletal muscle myocytes in vitro . Gene transcription in myocytes treated with 30 μmol/L sudachitin or vehicle for 48 h, normalized for β-actin (A). Data are means ± standard deviation. *P < 0.05 vs the indicated group. Closed bars: untreated cells; open bars: sudachitin-treated cells. The number of mitochondria in myocytes was increased by sudachitin (B, C). Myocytes were incubated with the fluorophore Mito-Tracker Green, which specifically labels mitochondria. Upper panel: control cells; lower panel: cells treated with 30 μmol/L sudachitin for 48 h. Arrows indicate stained mitochondria (B). Quantitative analysis of mitochondrial staining. Fluorescence intensity was measured by ImageJ software using the analyze particle function. *P < 0.05.