Figure 7
From: Curcumin and resveratrol inhibit nuclear factor-kappaB-mediated cytokine expression in adipocytes
Curcumin reduces TNFα, IL-1β, IL-6, COX-2 gene expression in differentiated adipocytes. For IL-1β, IL-6, and COX-2 expression analysis, differentiated adipocytes were incubated with TNFα and the indicated concentrations of curcumin for 62 h. Total RNA was extracted and gene expression levels were measured by qRT-PCR using the Comparative CT method. For TNFα expression analysis, differentiated adipocytes were incubated with curcumin in the absence of TNFα stimulation since we found that this stimulus has no effect on TNFα expression by adipocytes. For IL-1β, IL-6, and COX-2, relative expression levels were determined by comparing values obtained from TNFα-stimulated, curcumin-treated adipocytes to TNFα-stimulated, untreated adipocytes (assigned as 100% mRNA expression). Relative expression levels of TNFα were determined by comparing values obtained from curcumin-treated adipocytes to untreated adipocytes (assigned as 100% mRNA expression).