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Figure 1 | Nutrition & Metabolism

Figure 1

From: Effects of alpha-linolenic acid vs. docosahexaenoic acid supply on the distribution of fatty acids among the rat cardiac subcellular membranes after a short- or long-term dietary exposure

Figure 1

Specific protein markers of the different enriched fraction by Western-blotting. Membrane fraction were lysed in a RIPA buffer (see material and method for the composition) and 20 μg of the lysate was used for electrophoresis. Blots from Serca2 and Na/KATPase were made on the same membrane after 30 min of deshybridation at 50°C with a stripping buffer (100 mM mercapto-ethanol, 2% SDS and 62.5 mL Tris-HCl, pH 6.7). Cox-2 and Lamin A were made also on a same membrane. Western blot analysis shows single protein bands of 69 kDa, 85 kDa 115 kDa and 100 kDa for Lamine A, COX-2, Na/K ATPase and Serca2 respectively in rat heart fractions. Each specific protein band was observed in the specific fraction with a slight contamination of the NU in the MI fraction and a slight presence of the MI fraction in the NU fraction. TF, total fraction; NU, nucleus membrane fraction, CY, cytosolic fraction; MI, mitochondrial membrane fraction; SL, sarcolemmal membrane fraction; SR, sarcoplasmic reticulum fraction.

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Nutrition & Metabolism

ISSN: 1743-7075

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