Effects of alpha-linolenic acid vs. docosahexaenoic acid supply on the distribution of fatty acids among the rat cardiac subcellular membranes after a short- or long-term dietary exposure

Table 1 Formulation and fatty acid composition of the experimental diets.

	CTL diet	DHA diet	ALA diet	Extruded linseed flour⁵
	g/kg of diet	g/kg of diet	g/kg of diet	g/kg
Basal mix ¹
Protein				200
Soy protein isolate²	170	170	147
Glucides				110
Sucrose	220	220	216	35
Cornstarch	440	440	402
Fibers (mucilages, ...)				171
Cellulose	20	20		80
Minerals and other components				44
L-Cystine	5	5	5
Choline chloride	5	5	5
Mineral mixture³	50	50	48
Vitamin mixture³	10	10	10
Extruded linseed flour ⁴			122
Lipids				280
hydrogenated coconut oil⁵	15.2	15	11.3
Cocoa butter⁶	14.4	18	25.7
Sunflower seed oil⁷	48	17	8.9
Rapeseed oil⁸	2.4	10
n-3 LCPUFA-rich oil⁹		20
Humidity				80
Fatty acid composition ¹⁰	% of total FA	% of total FA	% of total FA	% of total FA
14:0	4.7	4.6	3.5	-
16:0	11.2	13.2	10.2	5.9
18:0	8.5	11.4	8.4	2.9
18:1 n-9	21.7	17.5	17.0	17.3
18:2 n-6	35.5	16.9	18.2	17.7
18:3 n-3	0.6	23.3	1.4	55.1
20:5 n-3	-	-	2.5	-
22:2 n-6	0.3	0.5	0.5	-
22:5 n-3	-	-	16.8	-
22:6 n-3	40.6	40.7	39.8	-
Total SFA	40.6	40.7	39.8	9.1
Total MUFA	22.7	18.4	18.0	18.1
Total PUFA	36.8	40.8	42.2	72.8
Total n-6 PUFA	36.0	17.5	20.5	17.7
Total n-3 PUFA	0.7	23.4	21.7	55.1
n-6/n-3 ratio	50.6	0.7	0.9	0.3
PUFA/SFA ratio	0.9	1.0	1.1	8.0

SFA. saturated fatty acids; MUFA. monounsaturated fatty acids. PUFA. polyunsaturated fatty acids; LCPUFA. long chain PUFA.
¹. The basal mix components in g/kg of diet were home-made as pellets by the UPAE (INRA). based on a formulation adapted from the AIN-93 (56).
². MP Biomedicals 905456.
³. Mineral AIN-93M and Vitamin AIN-93VX mixtures were prepared according to AIN-93 (56). MP Biomedicals.
⁴. Valomega 160™. Valorex. Combourtillé. France
⁵. Vegetaline. SCO. France.
⁶. Barry Callebaut. Meulan. France
⁷. Fruidor. Lesieur. Antony. France
⁸. Rustica. France
⁹. Long chain n-3 PUFA-enriched oil. V0137. Laboratoire Pierre Fabre. France
¹⁰. As determined by the analysis of the manufactured experimental diets and of the experimental extruded linseed flour.

ISSN: 1743-7075