Table 2 Quantification of [13C]AA, [13C]EPA and [13C]DHA after exposure of HepG2 cells to [13C] LA-/[13C]ALA-methylester at ratios of 0:1, 1:1, 4:1, 9:1 and 1:0 for 24 hours.
LA/ALA 0:1 | LA/ALA 1:1 | LA/ALA 4:1 | LA/ALA 9:1 | LA/ALA 1:0 | |
|---|---|---|---|---|---|
13 C LA administered [μmol] | - | 50 | 80 | 90 | 100 |
13 C AA [μmol] | - | 3.4 ± 0.04 | 5.7 ± 0.32 | 6.3 ± 0.21 | 3.3 ± 0.27 |
13 C ALA administered [μmol] | 100 | 50 | 20 | 10 | - |
13 C EPA [μmol] | 10.8 ± 0.51 | 5.7 ± 0.19 | 1.5 ± 0.05 | 1.4 ± 0.04 | - |
13 C DHA [μmol] | 0.18 ± 0.02 | 0.48 ± 0.01 | 0.11 ± 0.01 | 0.04 ± 0.004 | - |